Endopeptidase cleavage generates a functionally distinct isoform of
CTRP12 with an altered oligomeric state and signaling specificity
Adipose
tissue-derived adipokines are an important class of secreted metabolic
regulators that mediate tissue crosstalk to control systemic energy balance. We
recently described C1q/TNF-related protein-12 (CTRP12), a novel
insulin-sensitizing adipokine that regulates glucose metabolism in liver and
adipose tissue. However, the biochemical properties of CTRP12 and its naturally
occurring cleaved isoform have not been characterized. Here, we show that
CTRP12 is a secreted hormone subjected to multiple functionally relevant
posttranslational modifications at highly conserved residues. For example,
Asn-39 is glycosylated, while Cys-85 mediates the assembly of higher-order
oligomeric structure. Endopeptidase cleavage at Lys-91 generates a cleaved
globular gCTRP12 isoform, the expression of which is increased by insulin.
PCSK3/furin was identified as the major proprotein convertase expressed by
adipocytes that mediates the endogenous cleavage of CTRP12. Cleavage at Lys-91
is context dependent: mutation of the charged Arg-93 to Ala on the P2 position
enhanced cleavage, and triple mutations (K90A, K91A, R93A) abolished cleavage.
Importantly, the two isoforms of CTRP12 differ in oligomeric structures and are
functionally distinct. The full-length protein forms trimers and larger
complexes and the cleaved isoform consisted of predominantly dimers. While
full-length fCTRP12 strongly activated Akt signaling in H4IIE hepatocytes and
3T3-L1 adipocytes, gCTRP12 preferentially activated MAP kinase (Erk1/2 and
p38-MAPK) signaling. Further, only fCTRP12 improved insulin-stimulated glucose
uptake in adipocytes. These results reveal a novel mechanism controlling
signaling specificity and function of a hormone via cleavage-dependent
alteration in oligomeric state.
Wei Z, Lei X, Seldin M, Wong GW. . J Biol Chem. 2012 Aug 31. [Epub ahead
of print]
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Mouse/Rat
CTRP12 ELISA Kit
Code No.: SK00392-08
Size: 96 T
Price:
Standard range: 0.156-20 ng/ml
Sensitivity:50 pg/ml
Sample Type: serum, plasma
Sample require: 100 µL per well
Intra-CV:6-8%
Inter-CV: 8-12%
Protocol: PDF
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Human
CTRP12 ELISA Kit
Code No.: SK00392-06
Size: 96 T
Price:
Standard range: 0.049-200 ng/ml
Sensitivity:10 pg/ml
Sample Type: serum, plasma
Sample require: 100 µL per well
Intra-CV:6-8%
Inter-CV: 8-12%
Protocol: PDF
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Human
CTRP12 ELISA Kit
Code No.: SK00392-07
Size: 96 T
Price
Standard range: 0.64-2000ng/ml
Linear rage:5 ~ 500 ng/mL
Sensitivity:300 pg/ml
Sample Type: serum, plasma
Sample require: 100 µL per well
Intra-CV:6-8%
Inter-CV: 8-12%
Protocol: PDF
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Human
CTRP12/FAM132A Full Length Recombinant
Code No.: 00392-02-100
Size: 100 µg
Price
Protein ID: Q5T7M4
Gene ID:388581
MW:47.9 KD
Tag: His Tag on N-Terminus
Expressed: E. Coli
Purity: 95%
Data Sheet: PDF
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Human
CTRP12/FAM132AGlobular Form Recombinant
Code No.: 00392-01-100
Size: 100 µg
Price:
Protein ID: Q5T7M4
Gene ID:388581
MW:19.5 KD
Tag: His Tag on N-Terminus
Expressed: E. Coli
Purity: 95%
Data Sheet: PDF
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Mouse
CTRP12/FAM132A Full Length Recombinant
Code No.: 00392-03-100
Size: 100 µg
Price:
Protein ID: Q8R2Z0
Gene ID:67389
MW:48.9KD
Tag: His Tag on N-Terminus
Expressed: E. Coli
Purity: 95%
Data Sheet: PDF
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Mouse
CTRP12/FAM132A Globular Form Recombinant
Code No.: 00392-04-100
Size: 100 µg
Protein ID: Q8R2Z0
Gene ID:67389
MW:24.8 KD
Tag: His Tag on N-Terminus
Expressed: E. Coli
Purity: 95%
Data Sheet: PDF
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Anti-Human
CTRP12 IgG
Code No.: A00392-01-100
Size: 100 µg
Host: Rabbit
Antigen: Human CTRP12
Specificity: Human
Antibody Type: Purified IgG
Purification: Protein A affinity
Application: E, IHC
Data Sheet: PDF
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Anti-Mouse
CTRP12 IgG
Code No.: A00392-03-100
Size: 100 µg
Host: Rabbit
Antigen: Mouse CTRP12
Specificity: Mouse
Antibody Type: Purified IgG
Purification: Protein A affinity
Application: E, IHC
Data Sheet: PDF
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Name
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Catalog Number
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Size
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Price (USD)
|
ELISA Kit
|
CTRP12 (Mouse/Rat) ELISA Kit
|
SK00392-08
|
96T
|
|
CTRP12 (Human) ELISA Kit
|
SK00392-07
|
96T
|
|
CTRP12 (Human) ELISA Kit
|
SK00392-06
|
96T
|
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Recombinant
|
CTRP12/FAM132A (Human) Recombinant
|
00392-02-100
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100µg
|
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CTRP12/FAM132A Globular Form (Mouse) Recombinant
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00392-01-100
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100µg
|
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CTRP12 Full Length (Mouse) Recombinant
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00392-03-100
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100µg
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CTRP12 Globular Form (Mouse) Recombinant
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00392-04-100
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100µg
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Antibody
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Anti CTRP12 (Human) lgG Antibody
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A00392-01-100
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100µg
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Anti CTRP12 (Human) lgG Antibody, Biotinylated
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A00392-01-50B
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50µg
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Anti CTRP12 (Mouse) lgG Antibody
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A00392-03-100
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100µg
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Anti CTRP12 (Mouse) lgG Antibody, Biotinylated
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A00392-03-50B
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50µg
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Anti CTRP12 (Mouse) lgG Antibody
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A00392-04-100
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100µg
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Anti CTRP12 (Mouse) lgG Antibody, Biotinylated
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A00392-04-50B
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50µg
|
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