Molecular, biochemical and functional characterizations of C1q/TNF
family members: adipose-tissue-selective expression patterns, regulation by
PPAR-gamma agonist, cysteine-mediated oligomerizations, combinatorial
associations and metabolic functions
The
insulin-sensitizing hormone, adiponectin, belongs to the expanding C1q/TNF (tumor
necrosis factor) family of proteins. We recently identified a family of
adiponectin paralogues designated as CTRP (C1q/TNF-related protein) 1-7, and in
the present study describe CTRP10. In the present study, we show that CTRP1,
CTRP2, CTRP3, CTRP5 and CTRP7 transcripts are expressed predominantly by
adipose tissue. In contrast, placenta and eye expressed the highest levels of
CTRP6 and CTRP10 transcripts respectively. Expression levels of CTRP1, CTRP2,
CTRP3, CTRP6 and CTRP7 transcripts are up-regulated in 8-week-old obese (ob/ob)
mice relative to lean controls. Treatment of mice with a PPAR-gamma
(peroxisome-proliferator-activated receptor-gamma) agonist, rosiglitazone, increased
the expression of CTRP1 and decreased CTRP6 transcript levels. All CTRPs are
secreted glycoproteins when expressed in mammalian cells. CTRP1, CTRP2, CTRP3,
CTRP5 and CTRP6 circulate in the blood and are potential endocrine hormones;
their serum levels vary according to the sex and genetic background of mice.
Importantly, serum levels of CTRP1 and CTRP6 are increased in adiponectin-null
mice. Like adiponectin, all secreted CTRP proteins form trimers as their basic
structural units. CTRP3, CTRP5, CTRP6 and CTRP10 trimers are further assembled
into higher-order oligomeric complexes via disulfide bonding mediated by their
N-terminal cysteine residues. Besides forming homo-oligomers, CTRP1/CTRP6,
CTRP2/CTRP7 and adiponectin/CTRP2 are secreted as heterotrimers, thus providing
a mechanism to potentially generate functionally distinct ligands. Functional
characterization of one such family member, CTRP1, showed that it specifically
activates Akt and p44/42-MAPK (mitogen-activated protein kinase) signaling pathways
in differentiated mouse myotubes. Moreover, injection of recombinant CTRP1 into
mice significantly reduced their serum glucose levels. Thus at least CTRP1 may
be considered a novel adipokine. In summary, these molecular, biochemical and
functional data provide an important framework to further address the
physiological functions and mechanisms of the action of this family of secreted
glycoproteins in normal and disease states.
Wong GW , et al.Biochem J. 2008 Dec 1;416(2):161-77.
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Human
CTRP6 ELISA Kit
Code No.: SK00089-06
Size: 96 T
Price:
Standard range: 78-5000 pg/ml
Sensitivity: 10 pg/ml
Sample Type: serum
Sample require: 100 µL per well
Intra-CV:6-8%
Inter-CV: 8-10%
Protocol: PDF
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Mouse/Rat
CTRP6 ELISA Kit
Code No.: SK00089-08
Size: 96 T
Price:
Standard range: 156-5000 pg/ml
Sensitivity: 20 pg/ml
Sample Type: serum
Sample require: 100 µL per well
Intra-CV:6-8%
Inter-CV: 8-10%
Protocol: PDF
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Human
CTRP6/C1QTNF6 Globular Form Recombinant
Code No.: 00089-02-100
Size: 100 µg
Price:
Protein ID: Q9BXI9
Gene ID: 114904
MW:19.9 KD
Tag: His Tag on N-Terminus
Expressed: E. Coli
Purity: 95%
Data Sheet: PDF
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Human
CTRP6/C1QTNF6Full Length Recombinant
Code No.: 00089-01-100
Size: 100 µg
Price:
Protein ID: Q9BXI9
Gene ID: 114904
MW:29 KD
Tag: His Tag on N-Terminus
Expressed: E. Coli
Purity: 95%
Data Sheet: PDF
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Mouse
CTRP6/C1QTNF6Full Length Recombinant
Code No.: 00089-07-100
Size: 100 µg
Price:
Protein ID: Q6IR41
Gene ID: 72709
MW:44.2 KD
Tag: His Tag on N-Terminus
Expressed: E. Coli
Purity: 95%
Data Sheet: PDF
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Anti
CTRP6 (Mouse) IgG
Code No.: A00089-03-100
Size: 100 µg
Price:
Host: Rabbit
Antigen: Mouse CTRP6
Specificity: Mouse
Antibody Type: Purified IgG
Purification: Protein A affinity
Application: E, IHC
Data Sheet: PDF
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Anti
CTRP6 (Mouse)IgG
Code No.: A00089-07-100
Size: 100 µg
Price:
Host: Goat
Antigen: Mouse CTRP6
Specificity: Mouse, rat
Antibody Type: Purified IgG
Purification: Protein A affinity
Application: E, IHC
Data Sheet: PDF
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Mouse CTRP6/C1QTNF6 Globular Form
Recombinant
Code
No.: 00089-04-100
Size:
100 µg
Price:
Protein
ID: Q6IR41
Gene ID:
72709
MW: 33.1
KD
Tag: His
Tag on N-Terminus
Expressed:
E. Coli
Purity:
95%
Data
Sheet: PDF
Name
|
Catalog Number
|
Size
|
Price (USD)
|
ELISA
Kits
|
CTRP6/C1QTNF6 (Human) ELISA Kit
|
SK00089-06
|
96T
|
|
CTRP6/C1QTNF6 (Mouse/Rat) ELISA Kit
|
SK00089-08
|
96T
|
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Recombinant
|
CTRP6/C1QTNF6, Full Length (Human) Recombinant
|
00089-01-10
|
10µg
|
|
CTRP6/C1QTNF6, Full Length (Human) Recombinant
|
00089-01-50
|
50µg
|
|
CTRP6/C1QTNF6, Full Length (Human) Recombinant
|
00089-01-100
|
100µg
|
|
CTRP6/C1QTNF6, Full Length (Human) Recombinant
|
00089-01-1000
|
1mg
|
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CTRP6/C1QTNF6, Full Length (Human) Recombinant, Biotinylated
|
00089-01-50B
|
50µg
|
|
CTRP6/C1QTNF6, Globular Form (Human) Recombinant
|
00089-02-10
|
10µg
|
|
CTRP6/C1QTNF6, Globular Form (Human) Recombinant
|
00089-02-50
|
50µg
|
|
CTRP6/C1QTNF6, Globular Form (Human) Recombinant
|
00089-02-100
|
100µg
|
|
CTRP6/C1QTNF6, Globular Form (Human) Recombinant
|
00089-02-1000
|
1mg
|
|
CTRP6/C1QTNF6, Globular Form (Human) Recombinant, Biotinylated
|
00089-02-50B
|
50µg
|
|
CTRP6 Full Length (Mouse) Recombinant
|
00089-07-10
|
10µg
|
|
CTRP6 Full Length (Mouse) Recombinant
|
00089-07-100
|
100µg
|
|
CTRP6 Globular Form (Mouse) Recombinant
|
00089-04-10
|
10µg
|
|
CTRP6 Globular Form (Mouse) Recombinant
|
00089-04-100
|
100µg
|
|
Antibody
|
Anti CTRP6 (Human) lgG Antibody
|
A00089-01-100
|
100µg
|
|
Anti CTRP6 (Human) lgG Antibody, Biotinylated
|
A00089-01-50B
|
50µg
|
|
Anti CTRP6 (Mouse) lgG Antibody
|
A00089-03-100
|
100µg
|
|
Anti CTRP6 (Mouse) lgG Antibody, Biotinylated
|
A00089-03-50B
|
50µg
|
|
Anti CTRP6 (Mouse) lgG Antibody
|
A00089-04-100
|
100µg
|
|
Anti CTRP6 (Mouse) lgG Antibody, Biotinylated
|
A00089-04-50B
|
50µg
|
|
Goat Anti CTRP6 (Mouse) lgG Antibody
|
A00089-07-100
|
100µg
|
|
Goat Anti CTRP6 (Mouse) lgG, Biotinylated
|
A00089-07-50B
|
50µg
|
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