ErbB and HB-EGF signaling in heart development and function

The epidermal growth factor (EGF)-ErbB signaling network is composed of multiple ligands of the EGF family and four tyrosine kinase receptors of the ErbB family. In higher vertebrates, these four receptors bind a multitude of ligands. Ligandbinding induces the formation of various homo- and heterodimers of ErbB,potentially providing for a high degree of signal diversity. ErbB receptors andtheir ligands are expressed in a variety of tissues throughout development.Recent advances in gene targeting strategies in mice have revealed that theEGF-ErbB signaling network has fundamental roles in development, proliferation,differentiation, and homeostasis in mammals. The heparin-binding EGF-like growth factor (HB-EGF) is a member of the EGF family of growth factors that binds to andactivates the EGF receptor (EGFR/ErbB1) and ErbB4. Recent studies using severalmutant mice lacking HB-EGF expression have revealed that HB-EGF has a criticalrole in normal heart function and in normal cardiac valve formation inconjunction with ErbB receptors. HB-EGF signaling through ErbB2 is essential for the maintenance of homeostasis in the adult heart, whereas HB-EGF signalingthrough EGFR is required during cardiac valve development. In this review, weintroduce and discuss the role of ErbB receptors in heart function anddevelopment, focusing on the physiological function of HB-EGF in these processes.

Iwamoto R, Mekada E. Cell Struct Funct. 2006;31(1):1-14.

Diverse functions of HBEGF during pregnancy

The establishment of pregnancy requires an intimate physical interaction and a molecular dialogue between the conceptus and the maternal reproductive tract that commences at implantation and continues until the placenta is formed and fully functional. Failure of the regulatory processes that ensure the fidelity of this relationship can precipitate a catastrophic pregnancy loss. One of the earliest identified molecular mediators of blastocyst implantation is heparin-binding epidermal growth factor (EGF)-like growth factor (HBEGF), which signals between the endometrium and implanting trophoblast cells to synchronize their corresponding developmental programs. HBEGF expression by trophoblast cells of the developing placenta appears to regulate extravillous differentiation and provide cytoprotection in a sometimes-hostile environment. This versatile member of the EGF signaling system will be examined in light of its associations with key events during early pregnancy.

Jessmon P, Leach RE, Armant DR. Diverse functions of HBEGF during pregnancy. Mol Reprod Dev. 2009 Dec;76(12):1116-27.

Human Soluble HBEGF ELISA Kit

Code No.: SK00470-02

Size: 96 T

Standard range:312 -20000 pg/ml

Sensitivity: 70 pg/ml

Sample Type: serum, plasma

sample require: 50 µL per well

Intra-CV: 4-6%

Inter-CV: 8-10%

Data Sheet: PDF

Human HBEGF Recombinant

Code No.: 00470-01-100

Size: 100 µg

Protein ID: Q99075

Gene ID: 1839

MW: 9 KD

Tag: His Tag on N-Terminus

Expressed: E. Coli

Purity: 90%

Data Sheet: PDF

Anti Human HBEGF IgG

Code No.: A00470-01-100

Size: 100 µg

Host: Rabbit

Antigen: human HBEGF Rec.

Ab Type: Polyclonal IgG

Purification: Protein A

Applications: E, IHC, WB

Working Dilution: WB 1: 500 (2 ug/ml)

Data Sheet: PDF